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Differential Inhibition of Water and Ion Channel Activities

Aquaporin-1 (AQP1) is a major intrinsic protein that facilitates flux of water and other small solutes across cell membranes. In addition to its function as a water channel in maintaining fluid homeostasis, AQP1 also acts as a nonselective cation channel gated by cGMP, a property shown previously to facilitate rapid cell migration in a AQP1-expressing colon cancer cell line. Here we report two ...

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Architecture of the Saccharomyces cerevisiae NuA4/TIP60

The Mono Q peak fraction was diluted 8–12 times (20 mM HEPES [pH 8.0], 40 mM KOAc, 5 mM MgCl 2, 0.1% trehalose, 2 mM DTT, 0.01% NP-40) and applied to a …

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Standardizing chromatin research: a simple and universal

For the protocol comparison experiment, the same batch of fixed cells was used to test the following commonly used protocols: ‘BLUEPRINT’ , ‘Young’ , ‘ENCODE’ and NEXSON (this study). All samples were sonicated with the Covaris instrument to reach a fragment size distribution of 100–800 bp (Supplementary Figure S8).

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(PDF) Development of a Practical Method for Using Ozone Gas

Development of a Practical Method for Using Ozone Gas as a Virus Decontaminating Agent. ... Previous studies performed in a batch reactor have shown that the inactivation pathways through O3 and ...

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(PDF) Current Strategies of Polyploid Plant Genome Sequence

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